DOWNSTREAM pH CHANGES IN WATER FLOWING OVER THE GILLS OF RAINBOW TROUT
نویسنده
چکیده
We investigated the pH of interlamellar water of trout (Salmo gairdneri) by following changes in the downstream pH of expired water using a stopped-flow method. As water flowed over the gills of control fish, there was a significant decrease in water pH. Acetazolamide added to the water increased the CO2 disequilibrium, while carbonic anhydrase (CA) eliminated the CO2 disequilibrium relative to control water. Mucus excreted by the fish was found to contain CA activity by the pH-stat technique. We conclude water acidification is due to the conversion of excreted CO2 to HCO3~ and H + at the gill surface.
منابع مشابه
Respiratory control of arterial pH as temperature changes in rainbow trout Salmo gairdneri.
RANDALL, D. J., AND J. N. CAMERON. Respiratory control of arterial pH as temperature changes in rainbow trout Salmo gairdneri. Am. J. Physiol. 225(4) : 997-1002. 1973.-Arterial blood pH decreased as temperature increased, dpH/dT was 0.017 unit/“C and was independent of the acclimation history of the fish. Ventilation volume increased in proportion to oxygen uptake with temperature. Changes in w...
متن کاملProtective Effects of Dietary Spirulina platensis against Cadmium-Induced Oxidative Stress in Gills of Rainbow Trout
Background: Contamination of feeds with cadmium leads to oxidative stress in vital tissues such as gills and affects the fish survival. Therefore, an increase in the capacity of the antioxidant defense system and detoxification system of fish may reduce adverse effects of pollutants. This study investigated the protective effects of microalga Spirulina platensis against oxidative stress in gill...
متن کاملAcid-base Regulation following Exhaustive Exercise: a Comparison between Freshwater- and Sea Water-adapted Rainbow Trout (salmo Gairdneri)
Blood acid-base regulation following exhaustive exercise was investigated in freshwater(FW) and seawater(SW) adapted rainbow trout (Salmo gairdneri) of the same genetic stock. Following exhaustive exercise at 10°C, both FW and SW trout displayed a mixed respiratory and metabolic blood acidosis. However, in FW trout the acidosis was about double that of SW trout and arterial blood pH took twice ...
متن کاملThe Physiological Basis for Altered Na and Cl Movements across the Gills of Rainbow Trout (Oncorhynchus mykiss) in Alkaline (pH 5 9.5) Water
To test the hypothesis that internal ion imbalances at high pH are caused by altered branchial ion transporting capacity and permeability, radiotracers (Na and Cl) were used to measure ion movements across the gills of intact rainbow trout (Oncorhynchus mykiss) during 3 d exposure to pH 9.5. At control pH (pH 8.0), the trout were in net ion balance, but by 8 h at high pH, 60%–70% reductions in ...
متن کاملEffect of a traditional marinating on properties of rainbow trout fillet during chilled storage
In recent years, there has been an increasing interest in using food additives from natural sources to improve taste and also extend the shelf-life of semi-preserved foodstuffs. The aim of this study was to examine the chemical and microbiological changes promoted by a local marinating process in rainbow trout fillets during chilled storage. Fish fillets were immersed in marinades and stored at...
متن کامل